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Additional resources for Animal Cell Culture and Technology (THE BASICS (Garland Science))
Roller bottle systems offer the possibility of high yields of anchorage-dependent cells in replicate cultures. They were originally developed for the large-scale culture of anchorage-dependent cells used in the commercial production of viral vaccines. Equipment is available to accommodate up to 30 000 bottles each of 1 liter capacity. However, the process is labor intensive as each roller bottle must be handled individually for media changes or cell harvest. 11a Roller bottles of various sizes.
Courtesy of Chay Keogh, on behalf of Nikon UK Ltd. 8. 13). In general a centrifugal force of 150–200 g for 5–10 minutes should be sufficient to separate out cells from culture medium. Higher forces or longer times may cause damage to the cells by compacting them at the bottom of the centrifuge tube. Once centrifugation is completed, the supernatant should be decanted and the cells re-suspended. Typically swing-out buckets in a bench centrifuge will accommodate 50 ml or 15 ml plastic centrifuge tubes.
Samples of cell suspensions (~107 cells/ml) are offered for sale by the cell collections in frozen vials that can be transported in dry ice. On arrival the cells should be thawed and inoculated immediately into culture media or stored in liquid nitrogen. 1 lists a few examples of commonly used cell lines which can be obtained from the major international culture collections. All these cells grow well in culture when provided with an appropriate growth medium. The full history of each cell line is available from the collection catalogs as well as a list of references indicating previous use of the cells.