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Extra info for Applied Biochemistry and Bioengineering. Enzyme Technology
And Vas, K. (1968). Biotechnol. Bioeng. 10, 45. Production, Isolation, and Economics of Extracellular Enzymes Knud Aunstrup Novo Research Institute Bagsvaerd, Denmark I. Introduction II. History III. The Enzyme Industry A. Extracellular Microbial Enzymes for Industrial Use and the Producing Companies B. Market Situation C. Enzyme Costs IV. General Methods for Industrial Production of Extracellular Enzymes A. Production Strain B. Fermentation C. Recovery and Finishing V. Standardization and Control A.
Routine control o f enzyme products involves the following: • tests appropriate to the application, such as the absence o f transglucosidase in glucoamylase or the determination o f the protease level in amylase; • storage stability tests and analysis o f the concentration o f preserva tives and stabilizers such as C a ; • appearance tests o f color, odor, amount o f precipitate, and particle size; • tests for undesirable impurities such as heavy metals, mycotoxins, and antibiotic activity; • determination o f enzyme dust level in granulated enzyme preparations; •microbiological tests which usually involve a total viable count and tests for absence o f pathogens or indicator organisms such as coliforms.
A number o f enzyme Production of Extracellular Enzymes TABLE 31 I COMPANIES PRODUCING E X T R A C E L L U L A R ENZYMES F O R INDUSTRIAL U S E Denmark France Germany Great Britain Holland Japan Switzerland United States 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Grindstedvaerket A/S, Brabrand Novo Industri A/S, Bagsvaerd Soc. Rapidase, Seclin (subsidiary of No. d. Weser (subsidiary of No. 20). Rohm GmbH, Darmstadt ABM, Stockport, Cheshire Glaxo, Greenford, Middlesex Gist Brocades NV, Delft Amano, Nagoya Daiwa Kasei, Osaka Meiji Seika, Tokyo Meito Sangyo, Nagoya Nagase, Osaka Sankyo, Tokyo Shin Nippon, Tokyo Yakult Biochemicals, Nishinomiya Swiss Ferment AG (subsidiary of No.